New species of Nepenthes are being described on a somewhat regular basis as more remote areas of their range in southeast Asia are explored. Tissue culture is typically from seed, but it is also possible to propagate plants by meristem culture. This challenging technique requires a fair amount of plant material because contamination rates are high. Our lab uses either a liquid media on a rotator for small meristems (~2mm in size) or solid media for larger shoots as described below.
Chen J, P Gao and Z Gan. 2003. Tissue culture and rapid propagation of Nepenthes mirabilis. Plant Physiology Communications. 39:40.
Rasco Jr, ET and MAD Maquilan. 2005. Initial studies on in vitro germination and early seedling growth of Nepenthes truncata Macf. Carnivorous Plant Newsletter. 34:51-55.
Tandon P and TS Rathore. 1994. Mass clonal multiplication of the threatened Indian insectivorous plant (Nepenthes khasiana Hook F.) through shoot bud culture. In: Advances in Plant Tissue Culture in India: Proceedings of the XIII Plant Tissue Culture Conference, October 18-20, 1989, pp 185-192.
Zeng S, Z Chen and J Duan. 2003. Tissue culture and rapid propagation of Nepenthes x hybridus cv. Dyeriana. Plant Physiology newsletters. 39:479.